{"id":14904,"date":"2021-10-05T08:51:18","date_gmt":"2021-10-05T08:51:18","guid":{"rendered":"https:\/\/www.upjs.sk\/pracoviska\/tip\/sp\/casprot\/actualities\/25878-2\/"},"modified":"2023-03-28T13:18:56","modified_gmt":"2023-03-28T13:18:56","slug":"25878-2","status":"publish","type":"post","link":"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/","title":{"rendered":"Researchers have developed a matrix for one-step isolation of proteins by affinity chromatography"},"content":{"rendered":"

As part of the CasProt project, group of Assoc. Prof. Erik Sedl\u00e1k from the Center for Interdisciplinary Biosciences (CIB) from the Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice developed a matrix for one-step isolation of proteins by affinity chromatography in the CIB laboratories in cooperation with the group of Prof. Andreas Pl\u00fcckthun from the University of Zurich. The basis of the matrix is the protein DARPin (Designed Ankyrin Repetitive Protein) with high affinity for maltose-binding protein (MBP), which was modified by the method of rational design for the needs of affinity chromatography. In this work it was shown not only the high affinity of such a matrix for MBP conjugated proteins, but also the capacity and reproducibility of isolation in this way. This method was published in the International Journal of Biological Macromolecules (IF 6.853) (Nemergut M., \u0160krabana R., Berta M., Pl\u00fcckthun A., Sedl\u00e1k E. (2021). Purification of MBP fusion proteins using engineered DARPin affinity matrix<\/a>. J. Biol. Macromol. 187, 105-112.)<\/p>\n\n\n\n\n\n\n
\"\"<\/td>\nThe structure of the DARPin-MBP complex, which is the basis of the developed purification method, with marked lysines that have been modified by rational design (PDB: 1SVX and 1ANF).<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\n\u00a0<\/td>\n<\/tr>\n
Schematic representation of MBP-protein isolation by affinity chromatography with DARPin.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

As part of the CasProt project, group of Assoc. Prof. Erik Sedl\u00e1k from the Center for Interdisciplinary Biosciences (CIB) from the Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice developed a matrix for one-step isolation of proteins by affinity chromatography in the CIB laboratories in cooperation with the group of Prof. Andreas Pl\u00fcckthun from the University of … Continued<\/a><\/p>\n","protected":false},"author":1,"featured_media":14905,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"categories":[2],"tags":[39],"class_list":["post-14904","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized","tag-casprot"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"\nResearchers have developed a matrix for one-step isolation of proteins by affinity chromatography - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Researchers have developed a matrix for one-step isolation of proteins by affinity chromatography - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"As part of the CasProt project, group of Assoc. Prof. Erik Sedl\u00e1k from the Center for Interdisciplinary Biosciences (CIB) from the Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice developed a matrix for one-step isolation of proteins by affinity chromatography in the CIB laboratories in cooperation with the group of Prof. Andreas Pl\u00fcckthun from the University of … Continued\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\" \/>\n<meta property=\"og:site_name\" content=\"Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160\" \/>\n<meta property=\"article:published_time\" content=\"2021-10-05T08:51:18+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-28T13:18:56+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2022\/11\/obr1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"293\" \/>\n\t<meta property=\"og:image:height\" content=\"211\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"admin.wp\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin.wp\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\"},\"author\":{\"name\":\"admin.wp\",\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/#\/schema\/person\/cfcab55f75af7c1375335acad8f00717\"},\"headline\":\"Researchers have developed a matrix for one-step isolation of proteins by affinity chromatography\",\"datePublished\":\"2021-10-05T08:51:18+00:00\",\"dateModified\":\"2023-03-28T13:18:56+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\"},\"wordCount\":215,\"publisher\":{\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2022\/11\/obr1.jpg\",\"keywords\":[\"Casprot\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\",\"url\":\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/25878-2\/\",\"name\":\"Researchers have developed a matrix for one-step isolation of proteins by affinity chromatography - 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