{"id":20391,"date":"2025-10-29T13:57:31","date_gmt":"2025-10-29T13:57:31","guid":{"rendered":"https:\/\/www.upjs.sk\/pracoviska\/tip\/nova-analyticka-technika-hox-assay-prichadza-do-laboratoria-cib-tip-upjs\/"},"modified":"2025-10-29T15:45:24","modified_gmt":"2025-10-29T15:45:24","slug":"nova-analyticka-technika-hox-assay-prichadza-do-laboratoria-cib-tip-upjs","status":"publish","type":"post","link":"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/nova-analyticka-technika-hox-assay-prichadza-do-laboratoria-cib-tip-upjs\/","title":{"rendered":"New analytical technique HOX assay comes to the CIB, TIP-UPJ\u0160 laboratory"},"content":{"rendered":"\n<p>From October 6 to 17, 2025 RNDr. Ivana Timkov\u00e1, PhD., from the Center for Interdisciplinary Biosciences, TIP UPJ\u0160, completed a professional internship focused on mastering a new analytical technique that will soon be implemented in our laboratory.<\/p>\n\n\n<style>.kb-row-layout-id20391_6459ec-55 > .kt-row-column-wrap{align-content:start;}:where(.kb-row-layout-id20391_6459ec-55 > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}.kb-row-layout-id20391_6459ec-55 > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var(--global-kb-spacing-sm, 1.5rem);grid-template-columns:minmax(0, 1fr) minmax(0, 2fr);}.kb-row-layout-id20391_6459ec-55 > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){.kb-row-layout-id20391_6459ec-55 > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr) minmax(0, 2fr);}}@media all and (max-width: 767px){.kb-row-layout-id20391_6459ec-55 > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style><div class=\"kb-row-layout-wrap kb-row-layout-id20391_6459ec-55 alignnone wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-2-columns kt-row-layout-right-golden kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-top\">\n<style>.kadence-column20391_4d187e-4b > .kt-inside-inner-col,.kadence-column20391_4d187e-4b > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column20391_4d187e-4b > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column20391_4d187e-4b > .kt-inside-inner-col{flex-direction:column;}.kadence-column20391_4d187e-4b > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column20391_4d187e-4b > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column20391_4d187e-4b{position:relative;}@media all and (max-width: 1024px){.kadence-column20391_4d187e-4b > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column20391_4d187e-4b > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column20391_4d187e-4b\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image alignleft size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" src=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-3-768x1024.jpg\" alt=\"\" class=\"wp-image-20378\" style=\"width:315px;height:auto\" srcset=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-3-768x1024.jpg 768w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-3-225x300.jpg 225w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-3-1152x1536.jpg 1152w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-3.jpg 1280w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n<\/div><\/div>\n\n\n<style>.kadence-column20391_4adc48-02 > .kt-inside-inner-col,.kadence-column20391_4adc48-02 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column20391_4adc48-02 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column20391_4adc48-02 > .kt-inside-inner-col{flex-direction:column;}.kadence-column20391_4adc48-02 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column20391_4adc48-02 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column20391_4adc48-02{position:relative;}@media all and (max-width: 1024px){.kadence-column20391_4adc48-02 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column20391_4adc48-02 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column20391_4adc48-02\"><div class=\"kt-inside-inner-col\">\n<p>The HOX assay is an ultrasensitive fluorescent method used to detect halides and study enzymatic dehalogenation. It is based on the oxidation of halides by a vanadium-dependent chloroperoxidase from&nbsp;<em>Curvularia inaequalis<\/em>, producing hypohalous acids that are subsequently detected using aminophenyl fluorescein. Compared to existing methods, the HOX assay offers up to a thousandfold increase in sensitivity and can detect bromide ions at concentrations below one picomole.<\/p>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n<p>Under the guidance of Dr. Kova\u0159 (Loschmidt Laboratories, Brno, Czech Republic), Dr. Timkov\u00e1 was trained in the production of VCPO peroxidase and in the precise execution and optimization of the HOX assay for her specific dehalogenases.<\/p>\n\n\n\n<p>This internship was funded by the EU NextGenerationEU&nbsp;through the Recovery and Resilience Plan for Slovakia, under the project No. 09I03-03-V04-00112, and carried out in close collaboration with the project No. 09I02-03-V01-00021.<\/p>\n\n\n<style>.kb-row-layout-id20391_60f8ae-bd > .kt-row-column-wrap{align-content:start;}:where(.kb-row-layout-id20391_60f8ae-bd > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}.kb-row-layout-id20391_60f8ae-bd > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);grid-template-columns:repeat(2, minmax(0, 1fr));}.kb-row-layout-id20391_60f8ae-bd > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){.kb-row-layout-id20391_60f8ae-bd > .kt-row-column-wrap{grid-template-columns:repeat(2, minmax(0, 1fr));}}@media all and (max-width: 767px){.kb-row-layout-id20391_60f8ae-bd > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style><div class=\"kb-row-layout-wrap kb-row-layout-id20391_60f8ae-bd alignnone wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-2-columns kt-row-layout-equal kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-top\">\n<style>.kadence-column20391_b80877-23 > .kt-inside-inner-col,.kadence-column20391_b80877-23 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column20391_b80877-23 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column20391_b80877-23 > .kt-inside-inner-col{flex-direction:column;}.kadence-column20391_b80877-23 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column20391_b80877-23 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column20391_b80877-23{position:relative;}@media all and (max-width: 1024px){.kadence-column20391_b80877-23 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column20391_b80877-23 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column20391_b80877-23\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1-1024x768.jpg\" alt=\"\" class=\"wp-image-20372\" style=\"width:449px;height:auto\" srcset=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1-1024x768.jpg 1024w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1-300x225.jpg 300w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1-768x576.jpg 768w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1-1536x1152.jpg 1536w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-1.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div><\/div>\n\n\n<style>.kadence-column20391_f5a792-02 > .kt-inside-inner-col,.kadence-column20391_f5a792-02 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column20391_f5a792-02 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column20391_f5a792-02 > .kt-inside-inner-col{flex-direction:column;}.kadence-column20391_f5a792-02 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column20391_f5a792-02 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column20391_f5a792-02{position:relative;}@media all and (max-width: 1024px){.kadence-column20391_f5a792-02 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column20391_f5a792-02 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column kadence-column20391_f5a792-02\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-2-1024x768.jpg\" alt=\"\" class=\"wp-image-20375\" style=\"width:448px;height:auto\" srcset=\"https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-2-1024x768.jpg 1024w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-2-300x225.jpg 300w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-2-768x576.jpg 768w, https:\/\/www.upjs.sk\/app\/uploads\/sites\/24\/2025\/10\/technika-HOX-assay-2.jpg 1155w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>From October 6 to 17, 2025 RNDr. Ivana Timkov\u00e1, PhD., from the Center for Interdisciplinary Biosciences, TIP UPJ\u0160, completed a professional internship focused on mastering a new analytical technique that will soon be implemented in our laboratory. The HOX assay is an ultrasensitive fluorescent method used to detect halides and study enzymatic dehalogenation. It is &hellip; <a href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/nova-analyticka-technika-hox-assay-prichadza-do-laboratoria-cib-tip-upjs\/\">Continued<\/a><\/p>\n","protected":false},"author":16,"featured_media":20387,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"categories":[487,2],"tags":[],"class_list":["post-20391","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-apbc","category-uncategorized"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>New analytical technique HOX assay comes to the CIB, TIP-UPJ\u0160 laboratory - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/en\/nova-analyticka-technika-hox-assay-prichadza-do-laboratoria-cib-tip-upjs\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"New analytical technique HOX assay comes to the CIB, TIP-UPJ\u0160 laboratory - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"From October 6 to 17, 2025 RNDr. Ivana Timkov\u00e1, PhD., from the Center for Interdisciplinary Biosciences, TIP UPJ\u0160, completed a professional internship focused on mastering a new analytical technique that will soon be implemented in our laboratory. The HOX assay is an ultrasensitive fluorescent method used to detect halides and study enzymatic dehalogenation. 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The HOX assay is an ultrasensitive fluorescent method used to detect halides and study enzymatic dehalogenation. 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