{"id":13134,"date":"2018-09-17T10:49:33","date_gmt":"2018-09-17T10:49:33","guid":{"rendered":"https:\/\/www.upjs.sk\/pracoviska\/tip\/root\/cib\/zameranie\/"},"modified":"2025-06-13T10:17:24","modified_gmt":"2025-06-13T10:17:24","slug":"zameranie","status":"publish","type":"page","link":"https:\/\/www.upjs.sk\/pracoviska\/tip\/cib\/zameranie\/","title":{"rendered":"Zameranie"},"content":{"rendered":"\n<p><strong><a name=\"NAN\"><\/a><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">Nanomedic\u00edna<\/mark><\/strong><\/p>\n\n\n\n<p>Onkologick\u00e9 ochorenia predstavuj\u00fa z\u00e1va\u017en\u00fd zdravotn\u00fd, psychologick\u00fd a spolo\u010densk\u00fd probl\u00e9m. Vzh\u013eadom na starnutie popul\u00e1cie, zmeny \u017eivotn\u00e9ho \u0161t\u00fdlu a \u017eivotn\u00e9ho prostredia ich v\u00fdskyt neust\u00e1le narast\u00e1. V\u00fdskum v oblasti nanomedic\u00edny v CIB sa zameriava na zmiernenie negat\u00edvnych d\u00f4sledkov chemoterapie a zefekt\u00edvnenie protin\u00e1dorovej lie\u010dby prostredn\u00edctvom dvoch hlavn\u00fdch pr\u00edstupov:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>V\u00fdvoj selekt\u00edvneho nanotransportn\u00e9ho syst\u00e9mu pre cielen\u00fd transport lie\u010div:<\/strong> Cie\u013eom je vytvori\u0165 nanonosi\u010de schopn\u00e9 cielene dopravi\u0165 \u00fa\u010dinn\u00e9 l\u00e1tky priamo k n\u00e1dorov\u00fdm bunk\u00e1m. Tieto syst\u00e9my maj\u00fa vysok\u00fa afinitu k \u0161pecifick\u00fdm receptorom na povrchu n\u00e1dorov\u00fdch buniek a potenci\u00e1l pre personaliz\u00e1ciu lie\u010dby na z\u00e1klade individu\u00e1lnych mut\u00e1ci\u00ed pacientov.<\/li>\n\n\n\n<li><strong>Fotodynamick\u00e1 terapia (PDT):<\/strong> Sk\u00famaj\u00fa sa mechanizmy fotodynamicky navodenej bunkovej smrti s cie\u013eom optimalizova\u0165 t\u00fato terapeutick\u00fa met\u00f3du. V\u00fdskum prebieha na bunkovej \u00farovni, v modelovom syst\u00e9me chorioalantoickej membr\u00e1ne kuracieho embrya a in vivo na zvierac\u00edch modeloch, pri\u010dom cie\u013eom je vyvin\u00fa\u0165 protokoly aplikovate\u013en\u00e9 v klinickej praxi.<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">Prote\u00ednov\u00e9 in\u017einierstvo<\/mark><\/strong><\/p>\n\n\n\n<p>V\u00fdskumn\u00e1 oblas\u0165 prote\u00ednov\u00e9ho in\u017einierstva v CIB je zameran\u00e1 na v\u00fdvoj a charakteriz\u00e1ciu biomakromolek\u00fal \u2013 predov\u0161etk\u00fdm <strong>enz\u00fdmov a v\u00e4zbov\u00fdch prote\u00ednov <\/strong>\u2013 s nov\u00fdmi alebo vylep\u0161en\u00fdmi vlastnos\u0165ami pre aplik\u00e1cie v <strong>biomedic\u00edne, diagnostike, biotechnol\u00f3gii a syntetickej biol\u00f3gii.<\/strong> Pri v\u00fdvoji nov\u00fdch prote\u00ednov sa vyu\u017e\u00edvaj\u00fa modern\u00e9 technol\u00f3gie <strong>racion\u00e1lneho dizajnu<\/strong>, ako aj <strong>met\u00f3dy riadenej evol\u00facie<\/strong> vr\u00e1tane vysokopriepustn\u00e9ho skr\u00edningu, ribozomov\u00e9ho a kvasinkov\u00e9ho displeja.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">V\u00fdvoj stafylokin\u00e1zy ako trombolytick\u00e9ho agensu novej gener\u00e1cie<\/mark><\/strong><\/p>\n\n\n\n<p>Jednou z hlavn\u00fdch v\u00fdvojov\u00fdch l\u00edni\u00ed je in\u017einiering stafylokin\u00e1zy, bakteri\u00e1lneho prote\u00ednu s potenci\u00e1lom pre trombolytick\u00fa terapiu. Cie\u013eom je pomocou riadenej evol\u00facie a strukt\u00farneho dizajnu vytvori\u0165 varianty stafylokin\u00e1zy s:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>vy\u0161\u0161ou <strong>\u0161pecificitou vo\u010di fibr\u00ednu<\/strong> (na obmedzenie syst\u00e9mov\u00fdch ne\u017eiaducich \u00fa\u010dinkov),<\/li>\n\n\n\n<li><strong>zv\u00fd\u0161enou stabilitou <\/strong>v s\u00e9re,<\/li>\n\n\n\n<li><strong>zni\u017eenou imunogenicitou <\/strong>pre bezpe\u010dn\u00e9 klinick\u00e9 pou\u017eitie.<\/li>\n<\/ul>\n\n\n\n<p>Vyv\u00edjan\u00e9 stafylokin\u00e1zov\u00e9 kon\u0161trukty predstavuj\u00fa perspekt\u00edvne biologick\u00e9 lie\u010div\u00e1 pre ak\u00fatne trombotick\u00e9 stavy, ako s\u00fa infarkty a m\u0155tvice.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">In\u017einierstvo haloalk\u00e1nov\u00fdch dehalogen\u00e1z pre biokatal\u00fdzu a biosenzory<\/mark><\/strong><\/p>\n\n\n\n<p>CIB sa dlhodobo venuje <strong>prote\u00ednov\u00e9mu in\u017einierstvu haloalk\u00e1nov\u00fdch dehalogen\u00e1z <\/strong>\u2013 enz\u00fdmov schopn\u00fdch katalyzova\u0165 odstra\u0148ovanie halog\u00e9nov z organick\u00fdch zl\u00fa\u010den\u00edn. Vyu\u017e\u00edvaj\u00fa sa najm\u00e4 v oblastiach:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>biodegrad\u00e1cie environment\u00e1lnych polutanto<\/strong>v (napr. chl\u00f3rovan\u00e9 uh\u013eovod\u00edky),<\/li>\n\n\n\n<li><strong>v\u00fdvoja biosenzorov<\/strong> na detekciu environment\u00e1lnych kontaminantov,<\/li>\n\n\n\n<li><strong>biokatal\u00fdzy v organickej synt\u00e9ze.<\/strong><\/li>\n<\/ul>\n\n\n\n<p>Pomocou <strong>riadenej evol\u00facie<\/strong>, \u0161truktur\u00e1lnej anal\u00fdzy a modelovania enz\u00fdm-substr\u00e1tov\u00fdch interakci\u00ed sa vytv\u00e1raj\u00fa varianty s vylep\u0161enou \u0161pecificitou, termostabilitou a substr\u00e1tovou toleranciou.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">V\u00fdvoj fotosenzibiliz\u00e1torov na b\u00e1ze LOV2 dom\u00e9ny<\/mark><\/strong><\/p>\n\n\n\n<p>V r\u00e1mci <strong>optogenetiky a fotofarmakol\u00f3gie<\/strong> sa v CIB realizuje aj v\u00fdvoj <strong>fotosenzibiliz\u00e1torov zalo\u017een\u00fdch na LOV2 dom\u00e9ne<\/strong> (Light-Oxygen-Voltage domain 2), ktor\u00e1 je citliv\u00e1 na modr\u00e9 svetlo a umo\u017e\u0148uje kontrolu prote\u00ednovej aktivity pomocou osvetlenia. Cie\u013eom v\u00fdskumu je: <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>vytvori\u0165 <strong>modul\u00e1rne, svetlom ovl\u00e1date\u013en\u00e9 kon\u0161trukty<\/strong>, ktor\u00e9 umo\u017e\u0148uj\u00fa riadi\u0165 aktivitu f\u00fazovan\u00fdch terapeutick\u00fdch alebo sign\u00e1lnych prote\u00ednov,<\/li>\n\n\n\n<li><strong>regulova\u0165 intracelul\u00e1rne procesy<\/strong> (napr. apopt\u00f3zu, signaliz\u00e1ciu) svetlom v re\u00e1lnom \u010dase,<\/li>\n\n\n\n<li>navrhova\u0165 <strong>fotosenzibiliz\u00e1tory<\/strong> s optimalizovanou kinetikou n\u00e1vratu do tmav\u00e9ho stavu a s mo\u017enos\u0165ou zacielenia do vybran\u00fdch bunkov\u00fdch kompartmentov.<\/li>\n<\/ul>\n\n\n\n<p>Tieto v\u00fdskumn\u00e9 aktivity vytv\u00e1raj\u00fa z\u00e1klad pre v\u00fdvoj <strong>inteligentn\u00fdch biomolek\u00fal <\/strong>s kontrolovanou aktivitou, ktor\u00e9 n\u00e1jdu uplatnenie v <strong>prec\u00edznej medic\u00edne, biol\u00f3gii buniek aj syntetickej biol\u00f3gii.<\/strong><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-zakladna-modra-color\">Starnutie a oxida\u010dn\u00fd stres<\/mark><\/strong><\/p>\n\n\n\n<p>T\u00e1to v\u00fdskumn\u00e1 oblas\u0165 integruje \u0161t\u00fadium molekulovej bioenergetiky bunkov\u00e9ho d\u00fdchania a regul\u00e1cie oxida\u010dn\u00e9ho stresu. Sk\u00famaj\u00fa sa mechanizmy prenosu elektr\u00f3nov a prot\u00f3nov v mitochondri\u00e1ch, produkcia reakt\u00edvnych foriem kysl\u00edka, ako aj procesy bunkovej smrti spojen\u00e9 s dysfunkciou mitochondri\u00ed.<\/p>\n\n\n\n<p>V\u00fdsledky v\u00fdskumu prispievaj\u00fa k hlb\u0161iemu porozumeniu procesov starnutia a mechanizmov vzniku neurodegenerat\u00edvnych a onkologick\u00fdch ochoren\u00ed, \u010d\u00edm vytv\u00e1raj\u00fa predpoklady pre v\u00fdvoj nov\u00fdch terapeutick\u00fdch strat\u00e9gi\u00ed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Nanomedic\u00edna Onkologick\u00e9 ochorenia predstavuj\u00fa z\u00e1va\u017en\u00fd zdravotn\u00fd, psychologick\u00fd a spolo\u010densk\u00fd probl\u00e9m. Vzh\u013eadom na starnutie popul\u00e1cie, zmeny \u017eivotn\u00e9ho \u0161t\u00fdlu a \u017eivotn\u00e9ho prostredia ich v\u00fdskyt neust\u00e1le narast\u00e1. V\u00fdskum v oblasti nanomedic\u00edny v CIB sa zameriava na zmiernenie negat\u00edvnych d\u00f4sledkov chemoterapie a zefekt\u00edvnenie protin\u00e1dorovej lie\u010dby prostredn\u00edctvom dvoch hlavn\u00fdch pr\u00edstupov: Prote\u00ednov\u00e9 in\u017einierstvo V\u00fdskumn\u00e1 oblas\u0165 prote\u00ednov\u00e9ho in\u017einierstva v CIB je zameran\u00e1 &hellip; <a href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/cib\/zameranie\/\">\u010c\u00edta\u0165 \u010falej<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":13129,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"class_list":["post-13134","page","type-page","status-publish","hentry"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Zameranie - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/cib\/zameranie\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Zameranie - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"Nanomedic\u00edna Onkologick\u00e9 ochorenia predstavuj\u00fa z\u00e1va\u017en\u00fd zdravotn\u00fd, psychologick\u00fd a spolo\u010densk\u00fd probl\u00e9m. 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