{"id":22194,"date":"2026-09-09T12:43:26","date_gmt":"2026-09-09T12:43:26","guid":{"rendered":"https:\/\/www.upjs.sk\/pracoviska\/tip\/?p=22194"},"modified":"2026-09-09T12:47:35","modified_gmt":"2026-09-09T12:47:35","slug":"od-dna-k-novym-molekulam-letne-skoly-proteinoveho-inzinierstva-na-cib-tip-upjs","status":"publish","type":"post","link":"https:\/\/www.upjs.sk\/pracoviska\/tip\/od-dna-k-novym-molekulam-letne-skoly-proteinoveho-inzinierstva-na-cib-tip-upjs\/","title":{"rendered":"Od DNA k nov\u00fdm molekul\u00e1m: Letn\u00e9 \u0161koly prote\u00ednov\u00e9ho in\u017einierstva na CIB TIP UPJ\u0160"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Ako sa z DNA dostaneme k funk\u010dn\u00e9mu prote\u00ednu a ako mo\u017eno vlastnosti prote\u00ednov cielene meni\u0165 \u010di navrhova\u0165 nov\u00e9 molekuly? Odpovede na tieto ot\u00e1zky h\u013eadali \u0161tudenti po\u010das dvoch Letn\u00fdch \u0161k\u00f4l prote\u00ednov\u00e9ho in\u017einierstva, ktor\u00e9 sa v r\u00e1mci programu APBC uskuto\u010dnili na pracovisku Centra interdisciplin\u00e1rnych biovied TIP UPJ\u0160 v Ko\u0161iciach.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">K\u00fdm bakal\u00e1rski \u0161tudenti sa v druhom ro\u010dn\u00edku letnej \u0161koly&nbsp;<strong>Od DNA k prote\u00ednu<\/strong>&nbsp;obozn\u00e1mili so z\u00e1kladn\u00fdmi met\u00f3dami molekul\u00e1rnej biol\u00f3gie a prote\u00ednov\u00e9ho in\u017einierstva, pre \u0161tudentov magistersk\u00e9ho \u0161t\u00fadia bola tento rok po prv\u00fdkr\u00e1t pripraven\u00e1 nadv\u00e4zuj\u00faca letn\u00e1 \u0161kola&nbsp;<strong>V\u00fdvoj a dizajn nov\u00fdch molek\u00fal<\/strong>. T\u00e1 im umo\u017enila prenikn\u00fa\u0165 k pokro\u010dilej\u0161\u00edm met\u00f3dam a vysk\u00fa\u0161a\u0165 si postupy vyu\u017e\u00edvan\u00e9 pri v\u00fdvoji a optimaliz\u00e1cii prote\u00ednov.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Od DNA k prote\u00ednu: prv\u00e9 kroky v prote\u00ednovom in\u017einierstve<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Druh\u00e9ho ro\u010dn\u00edka Letnej \u0161koly prote\u00ednov\u00e9ho in\u017einierstva \u2013 Od DNA k prote\u00ednu sa z\u00fa\u010dastnilo desa\u0165 bakal\u00e1rskych \u0161tudentov. Po\u010das jedn\u00e9ho t\u00fd\u017ed\u0148a si mohli vysk\u00fa\u0161a\u0165, ako vyzer\u00e1 pr\u00e1ca v laborat\u00f3riu zameranom na prote\u00ednov\u00e9 in\u017einierstvo, a prepoji\u0165 poznatky zo \u0161t\u00fadia s vlastnou experiment\u00e1lnou pr\u00e1cou.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">O odborn\u00fd program a vedenie praktick\u00fdch cvi\u010den\u00ed sa starali&nbsp;<strong>Mgr. \u013dubo\u0161 Ambro, PhD.<\/strong>&nbsp;a&nbsp;<strong>RNDr. Michal Nemergut, PhD.<\/strong>, ktor\u00fdm v laborat\u00f3riu pom\u00e1hali&nbsp;<strong>RNDr. Alexandra Poliakova<\/strong>&nbsp;a&nbsp;<strong>RNDr. Ivana Timkov\u00e1, PhD.<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0160tudenti si postupne pre\u0161li z\u00e1kladn\u00fdmi met\u00f3dami molekul\u00e1rnej biol\u00f3gie \u2013 od pr\u00edpravy PCR reakcie cez nan\u00e1\u0161anie a kontrolu vzoriek pomocou agar\u00f3zovej g\u00e9lovej elektrofor\u00e9zy a\u017e po klonovanie. S\u00fa\u010das\u0165ou programu bola aj purifik\u00e1cia fluorescen\u010dn\u00fdch prote\u00ednov, zelen\u00e9ho GFP a \u010derven\u00e9ho RubyRed. Jednotliv\u00e9 experimenty tak \u0161tudentom umo\u017enili sledova\u0165 cestu od pr\u00e1ce s DNA a\u017e po z\u00edskanie v\u00fdsledn\u00e9ho prote\u00ednu.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>V\u00fdvoj a dizajn nov\u00fdch molek\u00fal: od z\u00e1kladov k riadenej evol\u00facii<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Novinkou tohto ro\u010dn\u00edka bola nadv\u00e4zuj\u00faca&nbsp;<strong>Letn\u00e1 \u0161kola prote\u00ednov\u00e9ho in\u017einierstva \u2013 V\u00fdvoj a dizajn nov\u00fdch molek\u00fal<\/strong>, ur\u010den\u00e1 \u0161tudentom magistersk\u00e9ho \u0161t\u00fadia. Jej cie\u013eom bolo roz\u0161\u00edri\u0165 ich vedomosti a predstavi\u0165 im pokro\u010dilej\u0161ie pr\u00edstupy vyu\u017e\u00edvan\u00e9 v s\u00fa\u010dasnom prote\u00ednovom in\u017einierstve.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Hlavn\u00fdmi t\u00e9mami boli&nbsp;<strong>riaden\u00e1 evol\u00facia<\/strong>&nbsp;a met\u00f3da&nbsp;<strong>kvasinkov\u00e9ho displeja (<em>yeast display<\/em>)<\/strong>. V teoretickej \u010dasti predstavila&nbsp;<strong>Mgr. M\u00e1ria Tomkov\u00e1, PhD.<\/strong>&nbsp;princ\u00edpy riadenej evol\u00facie,&nbsp;<strong>RNDr. M\u00e1ria Repovsk\u00e1, PhD.<\/strong>&nbsp;pribl\u00ed\u017eila technick\u00e9 aspekty kvasinkov\u00e9ho displeja a&nbsp;<strong>RNDr. Martin Menkyna<\/strong>&nbsp;sa venoval princ\u00edpom racion\u00e1lneho dizajnu prote\u00ednov.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Te\u00f3ria n\u00e1sledne pokra\u010dovala priamo v laborat\u00f3riu. Pod veden\u00edm M\u00e1rie Tomkovej a M\u00e1rie Repovskej a s pomocou doktorandky&nbsp;<strong>RNDr. Krist\u00edny Fel\u010d\u00edkovej<\/strong>&nbsp;si \u0161tudenti vysk\u00fa\u0161ali pr\u00edpravu kultiva\u010dn\u00fdch m\u00e9di\u00ed, zakladanie a preo\u010dkov\u00e1vanie kvasinkov\u00fdch kult\u00far. Pomocou fluorescen\u010dne aktivovan\u00e9ho triedi\u010da buniek (FACS) n\u00e1sledne vyberali kvasinkov\u00e9 bunky, ktor\u00e9 na svojom povrchu niesli sledovan\u00e9 prote\u00edny. Na vlastn\u00fdch experimentoch si tak mohli overi\u0165 princ\u00edpy, s ktor\u00fdmi sa predt\u00fdm obozn\u00e1mili v teoretickej \u010dasti programu.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Predn\u00e1\u0161ky, experimenty aj prezent\u00e1cia v\u00fdsledkov<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Prv\u00e9 dva dni oboch letn\u00fdch \u0161k\u00f4l doplnil spolo\u010dn\u00fd odborn\u00fd workshop. \u0160tudenti si po\u010das neho vypo\u010duli predn\u00e1\u0161ky odborn\u00edkov z praxe a z\u00edskali \u0161ir\u0161\u00ed poh\u013ead na praktick\u00e9 vyu\u017eitie poznatkov z oblasti biomedic\u00edny a proteomiky.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Intenz\u00edvny t\u00fd\u017ede\u0148 vyvrcholil v piatok z\u00e1vere\u010dn\u00fdmi prezent\u00e1ciami. \u0160tudenti zapojen\u00ed do programu V\u00fdvoj a dizajn nov\u00fdch molek\u00fal v skupin\u00e1ch predstavili priebeh svojej pr\u00e1ce, pou\u017eit\u00e9 experiment\u00e1lne postupy aj v\u00fdsledky, ku ktor\u00fdm po\u010das letnej \u0161koly dospeli.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Obe letn\u00e9 \u0161koly spojila spolo\u010dn\u00e1 my\u0161lienka \u2013 umo\u017eni\u0165 \u0161tudentom prekro\u010di\u0165 hranice teoretick\u00e9ho \u0161t\u00fadia a za\u017ei\u0165 vedeck\u00fa pr\u00e1cu priamo v laborat\u00f3riu. Od z\u00e1kladnej pr\u00e1ce s DNA a purifik\u00e1cie prote\u00ednov a\u017e po riaden\u00fa evol\u00faciu a selekciu buniek pomocou FACS si \u00fa\u010dastn\u00edci mohli vysk\u00fa\u0161a\u0165 met\u00f3dy, ktor\u00e9 s\u00fa s\u00fa\u010das\u0165ou modern\u00e9ho prote\u00ednov\u00e9ho in\u017einierstva.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Na z\u00e1ver si v\u0161etci \u00fa\u010dastn\u00edci prevzali certifik\u00e1ty o absolvovan\u00ed. Okrem nich si v\u0161ak odniesli predov\u0161etk\u00fdm nov\u00e9 vedomosti, praktick\u00e9 laborat\u00f3rne sk\u00fasenosti a konkr\u00e9tnej\u0161iu predstavu o tom, ako m\u00f4\u017ee vyzera\u0165 vedeck\u00e1 pr\u00e1ca a v\u00fdvoj nov\u00fdch molek\u00fal v praxi.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ako sa z DNA dostaneme k funk\u010dn\u00e9mu prote\u00ednu a ako mo\u017eno vlastnosti prote\u00ednov cielene meni\u0165 \u010di navrhova\u0165 nov\u00e9 molekuly? Odpovede na tieto ot\u00e1zky h\u013eadali \u0161tudenti po\u010das dvoch Letn\u00fdch \u0161k\u00f4l prote\u00ednov\u00e9ho in\u017einierstva, ktor\u00e9 sa v r\u00e1mci programu APBC uskuto\u010dnili na pracovisku Centra interdisciplin\u00e1rnych biovied TIP UPJ\u0160 v Ko\u0161iciach. K\u00fdm bakal\u00e1rski \u0161tudenti sa v druhom ro\u010dn\u00edku letnej &hellip; <a href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/od-dna-k-novym-molekulam-letne-skoly-proteinoveho-inzinierstva-na-cib-tip-upjs\/\">\u010c\u00edta\u0165 \u010falej<\/a><\/p>\n","protected":false},"author":16,"featured_media":22254,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[596],"tags":[595],"class_list":["post-22194","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-apbc-3","tag-apbc-2"],"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Od DNA k nov\u00fdm molekul\u00e1m: Letn\u00e9 \u0161koly prote\u00ednov\u00e9ho in\u017einierstva na CIB TIP UPJ\u0160 - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/pracoviska\/tip\/od-dna-k-novym-molekulam-letne-skoly-proteinoveho-inzinierstva-na-cib-tip-upjs\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Od DNA k nov\u00fdm molekul\u00e1m: Letn\u00e9 \u0161koly prote\u00ednov\u00e9ho in\u017einierstva na CIB TIP UPJ\u0160 - Technologick\u00fd a inova\u010dn\u00fd park | UPJ\u0160\" \/>\n<meta property=\"og:description\" content=\"Ako sa z DNA dostaneme k funk\u010dn\u00e9mu prote\u00ednu a ako mo\u017eno vlastnosti prote\u00ednov cielene meni\u0165 \u010di navrhova\u0165 nov\u00e9 molekuly? 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