{"id":9382,"date":"2012-03-13T09:01:02","date_gmt":"2012-03-13T09:01:02","guid":{"rendered":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-proteomiky\/"},"modified":"2022-09-12T20:45:26","modified_gmt":"2022-09-12T20:45:26","slug":"laboratorium-proteomiky","status":"publish","type":"page","link":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-proteomiky\/","title":{"rendered":"Laborat\u00f3rium proteomiky"},"content":{"rendered":"<p align=\"center\"><strong>\u0160pecializovan\u00e9 laborat\u00f3rium proteomiky<\/strong><br \/> \u00a0<\/p>\n<p style=\"text-align: justify\">Laborat\u00f3rium proteomiky Lek\u00e1rskej fakulty UPJ\u0160 (\u0160LP) pod veden\u00edm doc. RNDr. J\u00e1na Saba, CSc. je vybaven\u00e9 \u0161pi\u010dkov\u00fdm hmotnostn\u00fdm spektrometrom MALDI TOF\/TOF \u2013 Bruker Ultraflextreme, zbera\u010dom frakci\u00ed, tzv. spotterom \u2013 Bruker Proteineer fc II a pr\u00edstrojom na nan\u00e1\u0161anie matrice pre MALDI Imaging \u2013 Bruker ImagePrep. Na riadenie a\u00a0vyhodnocovanie meran\u00ed sa zak\u00fapil softv\u00e9r <em>Bruker Compass 1.3 for FLEX series<\/em>.<\/p>\n<p style=\"text-align: justify\">\u0160pi\u010dkov\u00e1 technika v celkovej hodnote viac ako 740 tis. \u20ac bola zak\u00fapen\u00e1 z prostriedkov projektu \u0160F Budovanie infra\u0161trukt\u00fary v centre excelentnosti SEPO-II (ITMS 26220120039).<\/p>\n<p style=\"text-align: justify\">Hmotnostn\u00fd spektrometer (obr. 1) sl\u00fa\u017ei na identifik\u00e1ciu prote\u00ednov prostredn\u00edctvom ur\u010denia ich hmotnosti, hmotnosti ich jednotliv\u00fdch peptidov a\u00a0ich fragmentov. Nameran\u00e1 hmotnostn\u00e9 spektr\u00e1 sa n\u00e1sledne porovn\u00e1vaj\u00fa so svetov\u00fdmi datab\u00e1zami hmotnostn\u00fdch spektier prote\u00ednov. V\u00fdsledkom je jednozna\u010dn\u00e1 identifik\u00e1cia analyzovan\u00e9ho prote\u00ednu. Spotter (obr. 2) je plne automatick\u00fd robot sl\u00fa\u017eiaci na at-line prepojenie MALDI TOF\/TOF hmotnostn\u00e9ho spektrometra so separa\u010dnou technikou. Konkr\u00e9tne sa jedn\u00e1 o\u00a0separa\u010dn\u00fa techniku vysoko \u00fa\u010dinnej kvapalinovej chromatografie (HPLC). Po HPLC separ\u00e1cii sa separovan\u00e9 peptidy nan\u00e1\u0161aj\u00fa na MALDI ter\u010d\u00edk s\u00a0nanesenou matricou. N\u00e1sledne je ter\u010d\u00edk so vzorkou vlo\u017een\u00fd do pr\u00edstroja,\u00a0analyzovan\u00fd a\u00a0vyhodnoten\u00fd rovnak\u00fdm sp\u00f4sobom ako pri MALDI TOF\/TOF meraniach. Na pr\u00edpravu vzorky tkan\u00edv, ktor\u00e9 maj\u00fa by\u0165 podroben\u00e9 MALDI anal\u00fdze, sa pou\u017e\u00edva pr\u00edstroj Bruker ImagePrep (obr. 3). Pr\u00edstroj rovnomerne nan\u00e1\u0161a extr\u00e9mne tenk\u00e9 vrstvy kry\u0161t\u00e1likov matrice a pomocou optick\u00fdch senzorov kontroluje stav nanesenej matrice, jej homogenitu a\u00a0hr\u00fabku vrstvy. Tak\u00e1to kontrola vrstvy nanesenej matrice je nevyhnutn\u00e1 pre dosiahnutie \u010do najlep\u0161ieho rozl\u00ed\u0161enia pri MALDI meran\u00ed. Softv\u00e9r <em>Bruker Compass 1.3 for FLEX series<\/em>\u00a0 je s\u00fabor programov nutn\u00fdch pre ovl\u00e1danie hmotnostn\u00e9ho spektrometra MALDI TOF\/TOF ako aj spottera a\u00a0r\u00f4znych vyhodnocovac\u00edch aplik\u00e1ci\u00ed (obr. 4).<\/p>\n<p style=\"text-align: justify\">\u0160pecializovan\u00e9 laborat\u00f3rium proteomiky je s\u00fa\u010das\u0165ou \u00dastavu lek\u00e1rskej biofyziky Lek\u00e1rskej fakulty UPJ\u0160 v\u00a0Ko\u0161iciach, ktor\u00e9 je lokalizovan\u00e9 v\u00a0budove \u00a0teoretick\u00fdch \u00fastavov na Triede SNP 1.<br \/> \u00a0<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" alt=\"\" border=\"0\" src=\"http:\/\/www.upjs.sk\/\/app\/uploads\/2022\/09\/loga-eu-spolu-6.jpg\" style=\"height:113px; width:490px\" \/><\/p>\n<p style=\"text-align: center\">\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0160pecializovan\u00e9 laborat\u00f3rium proteomiky \u00a0 Laborat\u00f3rium proteomiky Lek\u00e1rskej fakulty UPJ\u0160 (\u0160LP) pod veden\u00edm doc. RNDr. J\u00e1na Saba, CSc. je vybaven\u00e9 \u0161pi\u010dkov\u00fdm hmotnostn\u00fdm spektrometrom MALDI TOF\/TOF \u2013 Bruker Ultraflextreme, zbera\u010dom frakci\u00ed, tzv. spotterom \u2013 Bruker Proteineer fc II a pr\u00edstrojom na nan\u00e1\u0161anie matrice pre MALDI Imaging \u2013 Bruker ImagePrep. Na riadenie a\u00a0vyhodnocovanie meran\u00ed sa zak\u00fapil softv\u00e9r Bruker &hellip; <a href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-proteomiky\/\">\u010c\u00edta\u0165 \u010falej<\/a><\/p>\n","protected":false},"author":1,"featured_media":9385,"parent":3973,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Laborat\u00f3rium proteomiky - UPJS Kosice<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-proteomiky\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Laborat\u00f3rium proteomiky - UPJS Kosice\" \/>\n<meta property=\"og:description\" content=\"\u0160pecializovan\u00e9 laborat\u00f3rium proteomiky \u00a0 Laborat\u00f3rium proteomiky Lek\u00e1rskej fakulty UPJ\u0160 (\u0160LP) pod veden\u00edm doc. RNDr. J\u00e1na Saba, CSc. je vybaven\u00e9 \u0161pi\u010dkov\u00fdm hmotnostn\u00fdm spektrometrom MALDI TOF\/TOF \u2013 Bruker Ultraflextreme, zbera\u010dom frakci\u00ed, tzv. spotterom \u2013 Bruker Proteineer fc II a pr\u00edstrojom na nan\u00e1\u0161anie matrice pre MALDI Imaging \u2013 Bruker ImagePrep. 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