{"id":9477,"date":"2016-03-07T11:02:51","date_gmt":"2016-03-07T11:02:51","guid":{"rendered":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/"},"modified":"2016-03-07T11:02:51","modified_gmt":"2016-03-07T11:02:51","slug":"laboratorium-genomiky-a-transkriptomiky","status":"publish","type":"page","link":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/","title":{"rendered":"Laborat\u00f3rium genomiky a transkriptomiky"},"content":{"rendered":"<p style=\"text-align: justify;\">Pracovisko \u00daLB LF UPJ\u0160, kde sa Laborat\u00f3rium genomiky a transkriptomiky nach\u00e1dza, sa dlhodobo venuje v\u00fdskumu geneticky podmienenej predispoz\u00edcie jedincov pre vznik a rozvoj multifaktori\u00e1lnych ochoren\u00ed a \u0161t\u00fadiu geneticky podmienen\u00fdch rozdielov u jednotliv\u00fdch pacientov pri odpovedi na pr\u00edslu\u0161n\u00fa terapiu. Uveden\u00e9 Laborat\u00f3rium je preto zameran\u00e9 predov\u0161etk\u00fdm na anal\u00fdzu mut\u00e1ci\u00ed, g\u00e9nov\u00fdch polymorfizmov a expresie g\u00e9nov v s\u00favislosti so vznikom, diagnostikou a terapiou pr\u00edslu\u0161n\u00fdch multifaktor\u00e1lne podmienen\u00fdch ochoren\u00ed. Uvedenou problematikou sa pracovisko zaober\u00e1 v r\u00e1mci rozvinutej spolupr\u00e1ce s mnoh\u00fdmi \u00fastavmi a\u00a0klinick\u00fdmi pracoviskami UPJ\u0160.<\/p>\n<p style=\"text-align: justify;\">Laborat\u00f3rium genomiky a\u00a0transkriptomiky je z\u00a0h\u013eadiska pr\u00edstrojovej techniky vybaven\u00e9 \u00a0pr\u00edstrojov\u00fdm zariaden\u00edm na na detekciu a ur\u010dovanie zmien sekvencie nukleov\u00fdch kysel\u00edn (genetick\u00fd analyz\u00e1tor), robotickou stanicou na izol\u00e1ciu nukleov\u00fdch kysel\u00edn a\u00a0prote\u00ednov, robotickou stanicou na pipetovanie, pr\u00edstrojov\u00fdm vybaven\u00edm pre Real Time PCR a\u00a0HRM anal\u00fdzy, hlboko-mraziacimi, lamin\u00e1rnymi boxami a\u00a0pod. Unik\u00e1tne pr\u00edstrojov\u00e9 zariadenia\u00a0 boli zak\u00fapen\u00e9 z prostriedkov \u0160F E\u00da.<\/p>\n<p style=\"text-align: justify;\"><strong>Genetick\u00fdch analyz\u00e1tor GA 3500<\/strong> umo\u017e\u0148uje mimoriadne presn\u00e9 ur\u010denie jednotliv\u00fdch alternat\u00edv a zmien sekvencie DNA na z\u00e1klade sekvenovania, \u010di\u017ee ur\u010dovania poradia b\u00e1z. Dan\u00fd analyz\u00e1tor predstavuje nov\u00fd \u0161tandard na poli genetickej anal\u00fdzy syst\u00e9mom kapil\u00e1rnej elektrofor\u00e9zy. Osem-kapil\u00e1rny genetick\u00fd analyz\u00e1tor 3500 je mo\u017en\u00e9 okrem sekvenovania (muta\u010dn\u00e1 anal\u00fdza) pou\u017ei\u0165 i na fragmenta\u010dn\u00fa anal\u00fdzu. Softv\u00e9r 3500 zabezpe\u010d\u00ed komplexn\u00fa anal\u00fdzu z\u00edskan\u00fdch d\u00e1t. Dan\u00e9 inform\u00e1cie v\u00fdznamnou mierou napom\u00f4\u017eu exaktnej\u0161iemu pochopeniu mechanizmu \u00fa\u010dinku a d\u00f4sledkov mut\u00e1ci\u00ed pr\u00edslu\u0161n\u00fdch g\u00e9nov vyvol\u00e1vaj\u00facich pr\u00edslu\u0161n\u00e9 ochorenie.<\/p>\n<p style=\"text-align: justify;\"><strong>QuantStudio\u2122 12K Flex System s OpenArray\u2122 blokom a\u00a0 Accufill\u2122 syst\u00e9mom<\/strong><\/p>\n<p style=\"text-align: justify;\">Pr\u00edstroj umo\u017e\u0148uje vysoko citliv\u00fa anal\u00fdzu g\u00e9nov\u00fdch expresi\u00ed, profil\u00e1ciu miRNA, SNP genotypiz\u00e1ciu, high resolution melting (HRM) anal\u00fdzu, detekciu patog\u00e9nov, farmakogenomick\u00e9 aplik\u00e1cie, digit\u00e1lnu PCR. Digit\u00e1lna PCR predstavuje nov\u00fd pr\u00edstup detekcie raritn\u00fdch alel a\u00a0absol\u00fatnej kvantifik\u00e1cie\u00a0 nukleov\u00fdch kysel\u00edn pri pou\u017eit\u00ed minim\u00e1lneho mno\u017estva vzorky. U\u017e\u00edvate\u013e m\u00f4\u017ee pod\u013ea potreby meni\u0165\u00a0 bloky pod\u013ea po\u010dtu analyzovan\u00fdch vzoriek od 96 jamkov\u00e9ho form\u00e1tu, 384 jamkov\u00e9ho form\u00e1tu, 384 mikrofluidn\u00e9ho form\u00e1tu\u00a0 a\u017e po nanofluidn\u00fd OpenArray form\u00e1t umo\u017e\u0148uj\u00faci anal\u00fdzu a\u017e 12.000 d\u00e1t v\u00a0jednom behu. Na anal\u00fdzu m\u00f4\u017eu by\u0165 vyu\u017e\u00edvan\u00e9 preddefinovan\u00e9 eseje alebo je mo\u017en\u00e9 nadefinova\u0165 s\u00fabor esej\u00ed pod\u013ea svojich po\u017eiadaviek. Vybavenie zah\u0155\u0148a aj pr\u00edstroj Acuffil potrebn\u00fd na pr\u00edpravu vzoriek pre OpenArray form\u00e1t.<\/p>\n<p style=\"text-align: justify;\"><strong>Laborat\u00f3rium <\/strong><strong>genomiky a transkriptomiky<\/strong> je s\u00fa\u010das\u0165ou \u00dastavu lek\u00e1rskej biol\u00f3gie Lek\u00e1rskej fakulty UPJ\u0160 v\u00a0Ko\u0161iciach v\u00a0budove teoretick\u00fdch \u00fastavov na Triede SNP 1, sekcia II., 3 poschodie.<\/p>\n<p style=\"text-align: justify;\">\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pracovisko \u00daLB LF UPJ\u0160, kde sa Laborat\u00f3rium genomiky a transkriptomiky nach\u00e1dza, sa dlhodobo venuje v\u00fdskumu geneticky podmienenej predispoz\u00edcie jedincov pre vznik a rozvoj multifaktori\u00e1lnych ochoren\u00ed a \u0161t\u00fadiu geneticky podmienen\u00fdch rozdielov u jednotliv\u00fdch pacientov pri odpovedi na pr\u00edslu\u0161n\u00fa terapiu. Uveden\u00e9 Laborat\u00f3rium je preto zameran\u00e9 predov\u0161etk\u00fdm na anal\u00fdzu mut\u00e1ci\u00ed, g\u00e9nov\u00fdch polymorfizmov a expresie g\u00e9nov v s\u00favislosti so &hellip; <a href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\">\u010c\u00edta\u0165 \u010falej<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":3973,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"acf":{"acf_link_na_externy_obsah":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice\" \/>\n<meta property=\"og:description\" content=\"Pracovisko \u00daLB LF UPJ\u0160, kde sa Laborat\u00f3rium genomiky a transkriptomiky nach\u00e1dza, sa dlhodobo venuje v\u00fdskumu geneticky podmienenej predispoz\u00edcie jedincov pre vznik a rozvoj multifaktori\u00e1lnych ochoren\u00ed a \u0161t\u00fadiu geneticky podmienen\u00fdch rozdielov u jednotliv\u00fdch pacientov pri odpovedi na pr\u00edslu\u0161n\u00fa terapiu. Uveden\u00e9 Laborat\u00f3rium je preto zameran\u00e9 predov\u0161etk\u00fdm na anal\u00fdzu mut\u00e1ci\u00ed, g\u00e9nov\u00fdch polymorfizmov a expresie g\u00e9nov v s\u00favislosti so &hellip; \u010c\u00edta\u0165 \u010falej\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\" \/>\n<meta property=\"og:site_name\" content=\"UPJS Kosice\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/UniverzitaPavlaJozefaSafarikaVkosiciach\/\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@UPJS_kosice\" \/>\n<meta name=\"twitter:label1\" content=\"Predpokladan\u00fd \u010das \u010d\u00edtania\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 min\u00faty\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\",\"url\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\",\"name\":\"Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice\",\"isPartOf\":{\"@id\":\"https:\/\/www.upjs.sk\/#website\"},\"datePublished\":\"2016-03-07T11:02:51+00:00\",\"dateModified\":\"2016-03-07T11:02:51+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/#breadcrumb\"},\"inLanguage\":\"sk-SK\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.upjs.sk\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Univerzita\",\"item\":\"https:\/\/www.upjs.sk\/informacie\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Veda, v\u00fdskum a rozvoj\",\"item\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Inov\u00e1cie, rozvoj a eur\u00f3pska problematika\",\"item\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/\"},{\"@type\":\"ListItem\",\"position\":5,\"name\":\"N\u00e1rodn\u00e9 a univerzitn\u00e9 infra\u0161trukt\u00fary\",\"item\":\"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/\"},{\"@type\":\"ListItem\",\"position\":6,\"name\":\"Laborat\u00f3rium genomiky a transkriptomiky\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.upjs.sk\/#website\",\"url\":\"https:\/\/www.upjs.sk\/\",\"name\":\"UPJ\u0160 Ko\u0161ice\",\"description\":\"m\u00fadros\u0165 minulosti \u2013 poznatky pr\u00edtomnosti \u2013 vzdelanie bud\u00facnosti\",\"publisher\":{\"@id\":\"https:\/\/www.upjs.sk\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.upjs.sk\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"sk-SK\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.upjs.sk\/#organization\",\"name\":\"UPJ\u0160 Ko\u0161ice\",\"url\":\"https:\/\/www.upjs.sk\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"sk-SK\",\"@id\":\"https:\/\/www.upjs.sk\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.upjs.sk\/app\/uploads\/2022\/06\/LOGO-UPJS.png\",\"contentUrl\":\"https:\/\/www.upjs.sk\/app\/uploads\/2022\/06\/LOGO-UPJS.png\",\"width\":540,\"height\":125,\"caption\":\"UPJ\u0160 Ko\u0161ice\"},\"image\":{\"@id\":\"https:\/\/www.upjs.sk\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/UniverzitaPavlaJozefaSafarikaVkosiciach\/\",\"https:\/\/x.com\/UPJS_kosice\",\"https:\/\/www.instagram.com\/upjs_kosice\",\"https:\/\/www.linkedin.com\/company\/pavol-jozef-afrik-university-in-koice\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/","og_locale":"sk_SK","og_type":"article","og_title":"Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice","og_description":"Pracovisko \u00daLB LF UPJ\u0160, kde sa Laborat\u00f3rium genomiky a transkriptomiky nach\u00e1dza, sa dlhodobo venuje v\u00fdskumu geneticky podmienenej predispoz\u00edcie jedincov pre vznik a rozvoj multifaktori\u00e1lnych ochoren\u00ed a \u0161t\u00fadiu geneticky podmienen\u00fdch rozdielov u jednotliv\u00fdch pacientov pri odpovedi na pr\u00edslu\u0161n\u00fa terapiu. Uveden\u00e9 Laborat\u00f3rium je preto zameran\u00e9 predov\u0161etk\u00fdm na anal\u00fdzu mut\u00e1ci\u00ed, g\u00e9nov\u00fdch polymorfizmov a expresie g\u00e9nov v s\u00favislosti so &hellip; \u010c\u00edta\u0165 \u010falej","og_url":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/","og_site_name":"UPJS Kosice","article_publisher":"https:\/\/www.facebook.com\/UniverzitaPavlaJozefaSafarikaVkosiciach\/","twitter_card":"summary_large_image","twitter_site":"@UPJS_kosice","twitter_misc":{"Predpokladan\u00fd \u010das \u010d\u00edtania":"2 min\u00faty"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/","url":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/","name":"Laborat\u00f3rium genomiky a transkriptomiky - UPJS Kosice","isPartOf":{"@id":"https:\/\/www.upjs.sk\/#website"},"datePublished":"2016-03-07T11:02:51+00:00","dateModified":"2016-03-07T11:02:51+00:00","breadcrumb":{"@id":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/#breadcrumb"},"inLanguage":"sk-SK","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/laboratorium-genomiky-a-transkriptomiky\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.upjs.sk\/"},{"@type":"ListItem","position":2,"name":"Univerzita","item":"https:\/\/www.upjs.sk\/informacie\/"},{"@type":"ListItem","position":3,"name":"Veda, v\u00fdskum a rozvoj","item":"https:\/\/www.upjs.sk\/informacie\/vyskum\/"},{"@type":"ListItem","position":4,"name":"Inov\u00e1cie, rozvoj a eur\u00f3pska problematika","item":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/"},{"@type":"ListItem","position":5,"name":"N\u00e1rodn\u00e9 a univerzitn\u00e9 infra\u0161trukt\u00fary","item":"https:\/\/www.upjs.sk\/informacie\/vyskum\/inovacie-rozvoj-eu-problematika\/unikatne-pristroje\/"},{"@type":"ListItem","position":6,"name":"Laborat\u00f3rium genomiky a transkriptomiky"}]},{"@type":"WebSite","@id":"https:\/\/www.upjs.sk\/#website","url":"https:\/\/www.upjs.sk\/","name":"UPJ\u0160 Ko\u0161ice","description":"m\u00fadros\u0165 minulosti \u2013 poznatky pr\u00edtomnosti \u2013 vzdelanie bud\u00facnosti","publisher":{"@id":"https:\/\/www.upjs.sk\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.upjs.sk\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"sk-SK"},{"@type":"Organization","@id":"https:\/\/www.upjs.sk\/#organization","name":"UPJ\u0160 Ko\u0161ice","url":"https:\/\/www.upjs.sk\/","logo":{"@type":"ImageObject","inLanguage":"sk-SK","@id":"https:\/\/www.upjs.sk\/#\/schema\/logo\/image\/","url":"https:\/\/www.upjs.sk\/app\/uploads\/2022\/06\/LOGO-UPJS.png","contentUrl":"https:\/\/www.upjs.sk\/app\/uploads\/2022\/06\/LOGO-UPJS.png","width":540,"height":125,"caption":"UPJ\u0160 Ko\u0161ice"},"image":{"@id":"https:\/\/www.upjs.sk\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/UniverzitaPavlaJozefaSafarikaVkosiciach\/","https:\/\/x.com\/UPJS_kosice","https:\/\/www.instagram.com\/upjs_kosice","https:\/\/www.linkedin.com\/company\/pavol-jozef-afrik-university-in-koice\/"]}]}},"_links":{"self":[{"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/pages\/9477"}],"collection":[{"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/comments?post=9477"}],"version-history":[{"count":0,"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/pages\/9477\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/pages\/3973"}],"wp:attachment":[{"href":"https:\/\/www.upjs.sk\/wp-json\/wp\/v2\/media?parent=9477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}