Last Thursday, a successful demonstration webinar focused on computational enzyme engineering was held, attracting a diverse audience of researchers and academics. The online workshop was attended by approximately 40 participants. The event was held under the auspices of the APBC project.
The main highlights of the event were expert lectures delivered by H. J. Wijma and K. T. Korbeld from the University of Groningen. Their presentations covered the theoretical foundations of protein stability and strategies for improving it through engineering approaches.
The focus was placed on computational methods capable of predicting mutations that enhance the (thermo)stability of target proteins. The speakers introduced two advanced computational protocols, FRESCO and BoostMut, which have been specifically developed for this purpose. Participants gained not only theoretical insights but also practical guidance and useful tips on how to effectively apply these tools.
The workshop also included a live demonstration session, where the practical use of these computational pipelines was illustrated step by step. Detailed instructions for both protocols are available on GitHub, making them accessible to a broader community of researchers with basic training in computational methods.
Overall, the event significantly contributed to the dissemination of knowledge in the field of rational protein design and supported the integration of theoretical approaches with practical applications in modern biochemical research.
