Research at the department of medical and clinical biophysics is focused onto proteomic analysis of various samples. Laboratory is focused to shotgun analysis utilizing bottom-up approach but we are able to analyse whole protein samples by top-down analysis and tissues by mass spectrometry imaging. For peptide/protein separation different methods are applied utilizing one or two dimensional gel electrophoresis, off-gel fractionation or multidimensional HPLC.
Main area of interest are:
- Proteom analysis of breast cancer by four different approaches.
- Analysis of MCF7 breast cancer cell line
- Analysis of leukocytes isolated from the patients with breast cancer after each chemotherapy cycle
- Analysis of cancer tissues after surgery extraction
- Analysis of proteomic profile changes after application of non-ionizing electromagnetic field in combination with cytostatic and other biological treatment including immunomodulators
- Stem cells proteome analysis.
- Biomarker identification of differentiation, apoptosis and senescence.
- Influence of non-ionizing electromagnetic field onto differentiation process of stem cells.
- Inspection of stem cells for malignant transformation, which were transplanted to the patients with ischemic disease.
- Research focused on diagnostic potential of saliva proteome in relationship with systematic diseases and oral cavity diseases
- Research focused on changes in cerebrospinal fluid and platelets in suicidal patients
- Proteome analysis of leukocytes from patients with rheumatoid and juvenile arthritis.
Last update: 20.07.2020